Our studies suggest that the major role of the dense lamina is associated with the distribution stability and perhaps biogenesis of nuclear pore complexes. In addition several types of pore complex associated structures not previously reported by other electron microscope em techniques are observed by sem. Recent advances in light microscopy have extended the resolution to a scale allowing for the fine structure of the lamina to be imaged in the context of the whole.
The nuclear lamina consists of a dense fibrous meshwork of nuclear lamins type v intermediate filaments and is 14 nm thick according to recent cryo electron tomography studies. Springer nature is developing a new tool to find and evaluate protocols. Nuclear envelope nuclear pore complex scanning electron microscopy nuclear lamina this is a preview of subscription content log in to check access.
The nuclear lamina in necturus forms an orthogonal network with crossover distances which vary between 1 600 and 4 000 a and which may be related to the basic filament repeat of. The arrangement and interactions of npcs within amphibian nuclear envelopes have been studied using cryo electron microscopy of unfixed and frozen hydrated specimens. 1 division of cell biology mrc laboratory of molecular biology cambridge cb2 0qh united kingdom.
Cohen m 1 santarella r wiesel n mattaj i gruenbaum y. Electron microscopy of lamin and the nuclear lamina in caenorhabditis elegans. Elegans have helped establish lamins as essential components of nuclear architecture adding significant knowledge and helping understanding the functions of lamins and their associated proteins.
The electron microscopy techniques described above combined with the powerful genetic tools in c. We propose that these membranes are cleared of npcs because most npcs are attached to the still intact lamina at this stage and thus are held back while membranes are protruded by actin assembly. Our light and electron microscopy data clearly show that the f actin shell protrudes pore free nuclear membranes separating these from the lamina.
