The nuclear pore complex npc is a huge protein assemblage embedded in the nuclear envelope and has vital roles in gene expression by mediating transport between the nucleus and cytoplasm as well as regulating genome architecture strambio de castillia et al 2010 the npc is composed of seven substructures the transmembrane ring outer ring inner ring linker pore filaments nuclear. Here we present a single particle cryo electron microscopy cryo em structure of the cr from xenopus laevis npc at average resolutions of 5 5 7 9 å with local resolutions reaching 4 5 å. Nuclear pore complex npc exhibits structural plasticity and has only been characterized at local resolutions of up to 15 å for the cytoplasmic ring cr.
Although the structure of its scaffold is being revealed in increasing detail its transport functionality depends upon an assembly of intrinsically disordered proteins called fg nups anchored inside the pore s central channel which have hitherto eluded structural. The nuclear pore complex npc is a proteinaceous assembly that regulates macromolecular transport into and out of the nucleus. Christopher gained experience in the structural biology of the nuclear pore complex through his work in the hoelz laboratory at the california institute of technology as an amgen.
Burley and maria voigt during the spring of 2017. The nuclear pore complex educational resource was created by christopher markosian with the help of dr. It controls the traffic of proteins rna and rna proteins between nucleus and cytoplasm.
The nuclear pore complex npc is a large macro molecular assembly inserted into the nuclear envelope ne. Determining the path of single ribonucleoprotein rnp particles through the 100 nm wide nuclear pore complex npc by fluorescence microscopy remains challenging due to resolution limitation and. We use the periodic molecular structure of the nuclear pore complex to superimpose direct stochastic optical reconstruction.
Here we combine super resolution fluorescence localization microscopy with scanning electron microscopy to map the position of proteins of nuclear pore complexes in isolated xenopus laevis oocyte nuclear envelopes with molecular resolution in both imaging modes. A nuclear pore is a part of a large complex of proteins known as a nuclear pore complex that spans the nuclear envelope which is the double membrane surrounding the eukaryotic cell nucleus there are approximately 1 000 nuclear pore complexes npcs in the nuclear envelope of a vertebrate cell but it varies depending on cell type and the stage in the life cycle. Nucleoporins as components of the nuclear pore complex core structure and tpr as the architectural element of the nuclear basket.
High resolution field emission scanning electron microscopy of nuclear pore complex. Cell res 30 520 531 2020. Structure of the cytoplasmic ring of the xenopus laevis nuclear pore complex by cryo electron microscopy single particle analysis.
Huang g zhang y zhu x.
